Molecular studies
- In sugarcane, 10 genotypes were characterized using 27 morphological DUS descriptors. Among the 27 morphological descriptors, distinct morphological profiles were obtained for four out of ten genotypes. Five SSR markers were tested for molecular characterization of 10 sugarcane cultivars. Out of which, UGSM36 was dimorphic. And UGSM49 clearly separated the genotypes location wise
- Molecular characterization work was done for 15 rice cultivars revealed that SSR primer RM 320 and nine RAPD primers (OPH 04, OPH 08, PGG 08, OPH 05, OPB 14, OPG 13, OPC 04, OPH 1 and OPH 19) had the higher efficiency for DNA profiling and discrimination of rice culture. Among RAPD markers, OPH 19 primer gave higher polymorphism compared to others.
- In tomato SSR primers were tested for molecular characterization of 15 tomato cultivars, where three primers TOM 196, LEWPIG and SSR 304 were dimorphic. Primer LESSRPSPGa showed polymorphism specific for the genotype HN2.
Seed pre-sowing treatments
- In rice, seeds soaked in putrescine and spermidine 1 % for 16 h duration improved the field establishment and yield parameters under drought stress conditions.
- Rice seedlings raised after seed bio-priming with 1% phosphorous solubilizing bacteria + 1% K solubilizing bacteria + 2% moringa leaf extract and sprayed twice at 8 and 12 DAS with pink pigmented facultative methylotrops @ 1:100 recorded increased seedling vigour, growth and seed yield over unprimed seeds.
- Seed priming with 5 % grapes juice, improved the seed quality parameters and field emergence in paddy, greengram and groundnut.
- Seed priming with Chitosan 400 ppm + PPFM 2 % followed by foliar spraying of 2% DAP twice at flower initiation and pod formation stages increased the yield in black gram to the tune of 31 %.
- Seed priming treatments were standardized for sunflower and priming with Butylated hydroxy toluene@ 0.1 % has positive influence on enhancing the seedling growth attributes under unfavourable germination conditions (40°C +100 % RH).
Studies related to green manure crops
- In Kolingi cv. MDU 1 Seed production techniques were standardized. Under nutrient management fertilizers @ 25:50:10 kg NPK ha-1 with the plant spacing of 30 x 20 cm and foliar spray of pulse wonder twice at 50% flowering and 10 days later recorded higher plant growth and yield attributing characters. The seeds attained its physiological maturity at 35 days after anthesis. It is the best time for harvest of the seeds.
- Kolingi seeds graded with BSS 7/64 wire mesh sieve and pretreated with carbendazim @ 2g/kg before storage in 700 gauge polythene bag recorded highest germination and storability compared to untreated ones.
- In daincha the crop with indeterminate growth habit, Topping at 35 DAS along with application of K @ kg/ha and 2 % DAP spray at flowering twice recorded improved growth and yield attributes.
- Seed processing and testing procedures were standardized for sunn hemp, daincha and manila agathi as follows.
| Crop | Submitted sample size (g) | Working sample
Size (g) |
Days to
1st count |
Days to final count | Sieve size for processing | TZ test |
|---|---|---|---|---|---|---|
| Sunn hemp | 738 | 74 | 3 | 10 | round hole (10/64”) along with slotted sieve (1.8 mm) | 1% TZ for 3 h incubation
|
| Daincha | 483 | 48 | 5 | 7 | ||
| Manila agathi | 363 | 36 | – | – | round hole (7/64”) along with slotted sieve (1.8 mm) |
Studies related to Nutri-cereals
| Crop | Seed maturation | Foliar nutrition
(at panicle initiation and 50 % flowering) |
Threshing | Grading with wire mesh sieve |
|---|---|---|---|---|
| Proso millet | 35 DAA | Urea 1% +
ZnSO4 0.5% |
Paddy thresher | BSS 12 x 12 |
| Little millet | 40 DAA | Urea 1% +
ZnSO4 0.5% |
Manual beating | BSS 14 x 14 |
| Fox tail millet | 55 DAA | Humic acid 3 % | Paddy thresher | BSS 14 x 14 |
| Barnyard millet | 50 DAA | 2% horse gram sprout extract | Paddy thresher | BSS 14 x 14 |
Studies with Seaweed treatments
- In sunflower var. hybrid CO 2 seeds are treated with organic seaweed Sargassum myricocystum 5% extract for 10 h soaking followed by foliar spray at 5% during flowering stage is effective and also seeds treated with Sargassum myricocystum 5% could maintain the viability up to eight months of storage in polypropylene bag under ambient condition.
- Sesame cv. TMV 3 seeds treated with seaweed Caulera racemosa at 5% for 8 h soaking, and foliar spray (3%) given at vegetative and flowering stages were effective in growth and yield attributing parameters..
- Seed dry dressing with organic seaweed nano powder myricocystum@ 3 g kg-1 of seeds with 3 h shaking and soaking of seeds in S. myricocystum @ 1% for 3 h improved the physiological quality and biochemical parameters of Red gram CO (Rg) 7.
- In Kodo millet, foliar application of Sargassum myricocystum 5% extract at flowering and 50% flowering had positive effect on growth, yield and seed quality.
- In redgram APK 1 seed treatment with composted seaweed Sargassum myricocystum & cow dung slurry extract @ 5 % level along with foliar spray with the same extract at vegetative and flowering stages yielded better.