Hands on training in gene isolation, cloning and molecular characterization

The training is designed in such a way that participants will be able to perform gene cloning experiment independently.

The programme intends to impart hands on
training to the participants on the following:
• Plant genomic DNA isolation
• Quantification of DNA
• PCR primer designing
• RT PCR and PCR based gene amplification
• Plasmid isolation
• Restriction enzyme digestion
• Gene cloning – Ligation/transformation
• Molecular characterization of
rerecombinant clone [Restriction digestion/
S sequencing
• Lectures on basic and advanced gene
clcloning methods