Title of the Workshop
LC-MS Based Metabolomic approaches to identify signature bioactive compounds from natural sources for commercial applications
About the Workshop
The work shop on the LC-MS/MS will aid the trainees to gain knowledge about the basic principles of LC-MS, major components of MS, hard wares, soft ware components in LC-MS, different types of the ionization detectors, sample preparation, sample injection, positive and negative ionization modes, single quadrupole and triple quadrupole detectors. The trainees will also be trained in method development and data analysis for unknown samples. In addition to this, training will be imparted on the maintenance of the instrument and solutions to troubleshooting related to the column and detector.
The workshop on the LC-MS/MS is scheduled between 28.01.2023 and 29.01.2023 which comprises of the four lecture schedules on LC-MS basic principles, instrumentation and application, LC-MS/MS data interpretation and four hands on sessions on instrumentation of LC-MS, qualitative software, quantification basic, sensitivity and linearity, targeted MS and data processing, method development for unknown samples and maintenance of the LC-MS/MS instrument.
| Date | Time | Sessions | Presenter |
|---|---|---|---|
| 28.01.2023
Saturday
|
09.00 – 09.30 am | Inaugural Session | |
| 09.30 – 11.00 am | Lecture – I
Introduction to LC-MS/MS and its application (Online) |
Dr. Aditi Kundu
Senior Scientist, Division of Agricultural Chemicals, IARI, New Delhi |
|
| 11.15 – 12.45 pm | Lecture – II
Using Metabolomics in Crop Improvement(Online) |
Dr. Jyothilakshmi Vadassery,
Scientist V, NIPGR, New Delhi |
|
| 02.00 – 03.00 pm | Practical Session – I
Instrumentation of LC-MS, Introduction to qualitative software, Quantification basics, Sensitivity & linearity |
Mr. D. Venkatesh
Application Specialist, Spinco Biotech Coimbatore |
|
| 3.15 – 4.15 pm | Practical Session – II
Analysis of the standard (MS, MS/MS, Targeted MS) and data processing |
Mr. D. Venkatesh
Application Specialist, Spinco Biotech Coimbatore |
|
| 29.01.2023
Sunday |
09.30 – 11.00 am | Lecture – III
Metabolomic approaches for characterizing biochemical pathways (Online) |
Dr. Hetalkumar J.Panchal Professor Post Graduate Department of Biosciences Sardar Patel University Gujarat |
| 11.15 – 12.45 pm | Lecture – IV
Applications of LC-MS/MS for pesticide and other molecular detection and confirmation
|
Dr. A. Suganthi
Associate Professor, Department of Agricultural Entomology TNAU, Coimbatore |
|
| 02.00 – 03.00 pm | Practical Session – II
Method development and sample analysis
|
Mr. D. Venkatesh
Application Specialist, Spinco Biotech Coimbatore |
|
| 3.15 – 4.45 pm | Practical Session – II
Data processing and maintenance of LC-MS/MS |
Mr. D. Venkatesh
Application Specialist, Spinco Biotech Coimbatore |
|
| 05.00 – 05.30 pm | Valediction |
Date of the Workshop
28 & 29, JAN, 2023
Organizing Department
Biochemistry
Venue
Seminar Hall, Department of Biochemistry, CPMB&B, TNAU, Coimbatore
